anti human mouse fap biotin  (R&D Systems)

Journal: Cancer Science

Article Title: Evaluating near‐infrared photoimmunotherapy for targeting fibroblast activation protein‐α expressing cells in vitro and in vivo

doi: 10.1111/cas.15601

Figure Lengend Snippet: Verification of fibroblast activation protein alpha (FAP‐α) overexpression, and human and murine FAP‐α cross‐reactivity of AF3715. (A) Immunoblot analysis confirming FAP‐α overexpression in 231‐FAP and HT‐1080‐FAP cells. (B) Relative FAP‐α mRNA expression levels (mean ± SD) of prostate cancer‐associated fibroblasts (PCAFs), pancreatic cancer‐associated fibroblasts (CAF35), MDA‐MB‐231 (231), MDA‐MB‐231 (231‐FAP), HT‐1080, and HT‐1080‐FAP cells. *** p < 0.001. (C,D) Flow cytometry analyses confirming FAP‐α overexpression in (C) 231‐FAP and HT‐1080‐FAP cells with 231 and HT‐1080 cells used as controls and (D) CAF35 and PCAFs. (E) AF3715 binds human and murine FAP‐α with high affinity as shown by flow cytometry analysis of the binding of several anti‐FAP‐α Abs to 231‐FAP and 3T3‐FAP. AF3715 was compared with BMS168, MAB3715, and MAB9727. Mouse IgG isotype Ab was used as control

Article Snippet: Anti‐FAP‐α polyclonal sheep Ab, AF3715, monoclonal mouse Ab MAB3715, and monoclonal rat Ab MAB9729 were purchased from R&D Systems.

Techniques: Activation Assay, Over Expression, Western Blot, Expressing, Flow Cytometry, Binding Assay, Control